RT Journal Article SR Electronic T1 Bringing TrackMate in the era of machine-learning and deep-learning JF bioRxiv FD Cold Spring Harbor Laboratory SP 2021.09.03.458852 DO 10.1101/2021.09.03.458852 A1 Dmitry Ershov A1 Minh-Son Phan A1 Joanna W. Pylvänäinen A1 Stéphane U. Rigaud A1 Laure Le Blanc A1 Arthur Charles-Orszag A1 James R. W. Conway A1 Romain F. Laine A1 Nathan H. Roy A1 Daria Bonazzi A1 Guillaume Duménil A1 Guillaume Jacquemet A1 Jean-Yves Tinevez YR 2021 UL http://biorxiv.org/content/early/2021/09/03/2021.09.03.458852.abstract AB TrackMate is an automated tracking software used to analyze bioimages and distributed as a Fiji plugin. Here we introduce a new version of TrackMate rewritten to improve performance and usability, and integrating several popular machine and deep learning algorithms to improve versatility. We illustrate how these new components can be used to efficiently track objects from brightfield and fluorescence microscopy images across a wide range of bio-imaging experiments.Competing Interest StatementThe authors have declared no competing interest.