RT Journal Article
SR Electronic
T1 Treadmilling by FtsZ filaments drives peptidoglycan synthesis and bacterial cell division
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 077560
DO 10.1101/077560
A1 Alexandre W. Bisson Filho
A1 Yen-Pang Hsu
A1 Georgia R. Squyres
A1 Erkin Kuru
A1 Fabai Wu
A1 Calum Jukes
A1 Cees Dekker
A1 Seamus Holden
A1 Michael S. VanNieuwenhze
A1 Yves V. Brun
A1 Ethan C. Garner
YR 2016
UL http://biorxiv.org/content/early/2016/09/26/077560.abstract
AB How bacteria produce a septum to divide in two is not well understood. This process is mediated by periplasmic cell-wall producing enzymes that are positioned by filaments of the cytoplasmic membrane-associated actin FtsA and the tubulin FtsZ (FtsAZ). To understand how these components act in concert to divide cells, we visualized their movements relative to the dynamics of cell wall synthesis during cytokinesis. We find that the division septum is built at discrete sites that move around the division plane. Furthermore, FtsAZ filaments treadmill in circumferential paths around the division ring, pulling along the associated cell-wall-synthesizing enzymes. We show that the rate of FtsZ treadmilling controls both the rate of cell wall synthesis and cell division. The coupling of both the position and activity of the cell wall synthases to FtsAZ treadmilling guides the progressive insertion of new cell wall, synthesizing increasingly small concentric rings to divide the cell.One-sentence summary Bacterial cytokinesis is controlled by circumferential treadmilling of FtsAZ filaments that drives the insertion of new cell wall.