RT Journal Article
SR Electronic
T1 A Novel Optical Design Enabling Lightweight and Large Field-Of-View Head-Mounted Microscopes
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.09.03.458947
DO 10.1101/2021.09.03.458947
A1 J. R. Scherrer
A1 Galen F. Lynch
A1 Jie J. Zhang
A1 Michale S. Fee
YR 2021
UL http://biorxiv.org/content/early/2021/09/06/2021.09.03.458947.abstract
AB We present a novel fluorescence microscope light path that enables imaging of over a thousand neurons in freely-behaving mice and imaging in juvenile songbirds. The light path eliminates traditional illumination optics, allowing for head-mounted microscopes that have both a lower weight and a larger field-of-view (FOV) than previously possible. Using this light path, we designed two microscopes: one optimized for field-of-view (∼4 mm FOV; 1.4 g), and the other optimized for weight (1.0 mm FOV; 1.0 g).Competing Interest StatementJ.R.S., G.F.L., and M.S.F. are listed as inventors on a patent application for this technology.