RT Journal Article
SR Electronic
T1 CardIAP: Calcium images analyzer web application
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.09.08.452919
DO 10.1101/2021.09.08.452919
A1 Ana Julia Velez Rueda
A1 Agustín García Smith
A1 Luis Alberto Gonano
A1 Maria Silvina Fornasari
A1 Gustavo Parisi
A1 Leandro Matías Sommese
YR 2021
UL http://biorxiv.org/content/early/2021/09/09/2021.09.08.452919.abstract
AB Motivation Ionic calcium (Ca2+) plays the role of the second messenger in eukaryotic cells associated with cellular functions of regulation of the cell cycle, such as transport, motility, gene expression, and metabolism (Permyakov and Kretsinger, 2009). The use of fluorometric techniques in isolated cells, loaded with Ca2+ sensitive fluorescent probes allows the quantitative measurement of dynamic events that occur in living, functioning cells. The Cardiomyocytes Images Analyzer Application (CardIAP) covers the need for tools to analyze and retrieve information from confocal microscopy images, in a systematic, accurate, and fast way.Results Here we present the CardIAP web app, an automated method for the identification of spatio-temporal patterns in a calcium fluorescence imaging sequence. Through this tool, users can analyze single or multiple Ca2+ transients from confocal line-scan images and obtain quantitative information on the dynamic response of the stimulated myocyte.Our web application also allows the user the extraction of data on calcium dynamics in downloadable tables and plots, simplifying the calculation of the alternation and discordance indices and their classification. CardIAP could assist in studying the underlying mechanisms of anomalous calcium release phenomena.Availability and implementation CardIAP is an open-source app, entirely developed in Python, which can be freely accessed and used at http://cardiap.herokuapp.com/.Competing Interest StatementThe authors have declared no competing interest.