RT Journal Article SR Electronic T1 Lipid chaperone LBP-8 coordinates with nuclear factors to promote longevity in Caenorhabditis elegans JF bioRxiv FD Cold Spring Harbor Laboratory SP 2021.09.09.459489 DO 10.1101/2021.09.09.459489 A1 Jonathon Duffy A1 Qinghao Zhang A1 Sung Y. Jung A1 Meng C. Wang YR 2021 UL http://biorxiv.org/content/early/2021/09/09/2021.09.09.459489.abstract AB Eukaryotic cells are composed of a variety of organelles. Their coordination plays crucial roles in cellular homeostasis and organism longevity and is mediated by proteins with the ability to transport between different organelles. In Caenorhabditis elegans, LBP-8 is a pro-longevity lipid chaperone that can localize to both lysosomes and the nucleus. Here we profiled LBP-8’s binding partners using immunoprecipitation and mass spectrometry. From the 45 identified candidates, we discovered four nuclear factors that are required for the LBP-8-induced longevity. Among them, RPC-2, an RNA Polymerase III core subunit, is also necessary for the nuclear localization of LBP-8. Moreover, we have screened nuclear transport machinery components, and revealed the requirement of the nuclear import, not export, for the LBP-8 longevity effects. Together, these results suggest that the lipid chaperone LBP-8 relies on specific nuclear factors to retain in the nucleus and regulate longevity.Competing Interest StatementThe authors have declared no competing interest.