PT  - JOURNAL ARTICLE
AU  - Ioannis Nellas
AU  - K. Venkatesan Iyer
AU  - Juan M. Iglesias-Artola
AU  - André Nadler
AU  - Natalie A. Dye
AU  - Suzanne Eaton
TI  - Hedgehog signaling can enhance glycolytic ATP production in the <em>Drosophila</em> wing disc
AID  - 10.1101/2021.09.11.459911
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.09.11.459911
4099  - http://biorxiv.org/content/early/2021/09/12/2021.09.11.459911.short
4100  - http://biorxiv.org/content/early/2021/09/12/2021.09.11.459911.full
AB  - Energy production and utilization is critically important for animal development and growth. How it is regulated in space and time during tissue growth remains largely unclear. Toward this end, we used a FRET-based adenosine triphosphate (ATP) sensor to dynamically monitor ATP levels across a growing tissue, using the Drosophila wing disc. We discovered that steady-state levels of ATP are spatially uniform across the wing pouch. Pharmacologically inhibiting oxidative phosphorylation, however, reveals spatial heterogeneities in metabolic behavior, whereby signaling centers at compartment boundaries produce more ATP from glycolysis than the rest of the tissue. Genetic perturbations indicate that the conserved Hedgehog (Hh) signaling pathway can enhance ATP production by glycolysis. Collectively, our work reveals a positive feedback loop between Hh signaling and energy metabolism, advancing our understanding of the connection between conserved developmental patterning genes and energy production during animal tissue development.Competing Interest StatementThe authors have declared no competing interest.