RT Journal Article
SR Electronic
T1 Visualizing synaptic plasticity in vivo by large-scale imaging of endogenous AMPA receptors
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.03.01.972216
DO 10.1101/2020.03.01.972216
A1 Austin R. Graves
A1 Richard H. Roth
A1 Han L. Tan
A1 Qianwen Zhu
A1 Alexei M. Bygrave
A1 Elena Lopez-Ortega
A1 Ingie Hong
A1 Alina C. Spiegel
A1 Richard C. Johnson
A1 Joshua T. Vogelstein
A1 Daniel J. Tward
A1 Michael I. Miller
A1 Richard L. Huganir
YR 2021
UL http://biorxiv.org/content/early/2021/09/13/2020.03.01.972216.abstract
AB Elucidating how synaptic molecules such as AMPA receptors mediate neuronal communication and tracking their dynamic expression during behavior is crucial to understand cognition and disease, but current technological barriers preclude large-scale exploration of molecular dynamics in vivo. We have developed a suite of innovative methodologies that break through these barriers: a new knockin mouse line with fluorescently tagged endogenous AMPA receptors, two-photon imaging of hundreds of thousands of labeled synapses in behaving mice, and computer-vision-based automatic synapse detection. Using these tools, we can longitudinally track how the strength of synapses changes during behavior. We used this approach to generate an unprecedentedly detailed spatiotemporal map of synaptic plasticity underlying sensory experience. More generally, these tools can be used as an optical probe capable of measuring functional synapse strength across entire brain areas during any behavioral paradigm, describing complex system-wide changes with molecular precision.Competing Interest StatementThe authors have declared no competing interest.