RT Journal Article
SR Electronic
T1 MyD88 Mediates Colitis- and RANKL-induced Microfold Cell Differentiation
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.09.16.460646
DO 10.1101/2021.09.16.460646
A1 Yang Li
A1 Shanshan Yang
A1 Xin Huang
A1 Ning Yang
A1 Caiying Wang
A1 Jing Zhao
A1 Zhizhong Jing
A1 Luc Willems
A1 Guangliang Liu
YR 2021
UL http://biorxiv.org/content/early/2021/09/16/2021.09.16.460646.abstract
AB Intestinal microfold (M) cells are critical for sampling antigen in the gut and initiating the intestinal mucosal immune response. In this study, we found that the differentiation efficiency of M cells was closely related to the colitis severity. The expression levels of M cells differentiation-related genes were synchronized with the kinetics of pro-inflammatory cytokines expression originated from dextran sulfate sodium (DSS) induction and Salmonella infection. Compared with wild-type (WT) mice, MyD88-/- mice exhibited significantly lower expression levels of M cells differentiation-related genes. However, DSS could induce colitis in MyD88-/- mice but failed to promote M cells differentiation. Furthermore, the receptor activator of the Nuclear Factor-κB ligand (RANKL) induced M cells differentiation in murine intestinal organoids prepared from both WT and MyD88-/- mice. However, less M cells differentiation were found in MyD88-/- mice as compared with WT mice. Hence, we concluded that myeloid differentiation factor 88 (MyD88) is an essential molecule for colitis- and RANKL-related M cells differentiation.Competing Interest StatementThe authors have declared no competing interest.AbbreviationsCCL9C-C motif ligand 9DSSdextran sulfate sodiumFAEfollicle-associated epitheliumGP2glycoprotein 2Lgr5leucine-rich repeat-containing G-protein coupled receptor 5M cellsmicrofold cellsMyD88myeloid differentiation factor 88NALTnasopharynx-associated lymphoid tissueRANKreceptor activator of NF-κBRANKLreceptor activator of the Nuclear Factor-κB ligandTNF-αtumor necrosis factor-αTRAF6tumor necrosis factor receptor-associated factor 6