RT Journal Article
SR Electronic
T1 Bacterial composition reflects fine-scale salinity changes while phylogenetic diversity exhibits a strong salt divide
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.09.14.460410
DO 10.1101/2021.09.14.460410
A1 Ariane L. Peralta
A1 Mario E. Muscarella
A1 Alexandra Stucy
A1 Jo A. Werba
A1 Michael W. McCoy
YR 2021
UL http://biorxiv.org/content/early/2021/09/17/2021.09.14.460410.abstract
AB Climate change induced salinization events are predicted to intensify and lead to increased salt stress in freshwater aquatic ecosystems. As a consequence, formerly distinct abiotic conditions and associated biotic communities merge, and the emergence, loss, and persistence of microbial taxa modify the types and rates of ecosystem processes. This study examined how bacterial taxonomic and phylogenetic diversity and ecosystem function respond to acute salinization events where freshwater and estuarine communities and environments coalesce. We hypothesize that if the salinity change outpaces microbial adaptation or saline microbial populations are not yet established in formerly freshwater conditions, then we predict diminished carbon cycling rates, decreased microbial diversity, and altered the composition of microbial communities compared to historically freshwater communities. We used an experimental mesocosm approach to determine how salinity and the merging of distinct communities influenced resultant bacterial community structure and function. Each mesocosm represented different salinities (0, 5, 9, 13 psu). Two dispersal treatments, representing aquatic communities sourced from brackish 13 psu ponds and a mix of 13 psu and freshwater ponds, were added to all salinity levels and replicated four times. Results revealed that salinity, but not dispersal, decreased bacterial taxonomic and phylogenetic diversity. Carbon mineralization rates were highest in freshwater conditions and associated with low relative abundance indicator taxa. Acute salinity changes, such as localized flooding due to storm surge, will more negatively affect freshwater aquatic communities compared to chronic exposure to salinization where the communities have had time to adapt or turnover resulting in recovered biogeochemical functions.IMPORTANCE STATEMENT Climate change induced salinization results in the mixing of formerly distinct environmental conditions and aquatic communities. This study examined the consequence of short-term, acute salinity stress on aquatic bacterial taxonomic and phylogenetic diversity and ecosystem function using an experimental approach. Results revealed that salinity, but not the source of aquatic communities, decreased bacterial taxonomic and phylogenetic diversity. Carbon mineralization rates, which represented ecosystem function, were highest in freshwater conditions and also associated with low relative abundance indicator bacterial taxa. Taken together, acute salinity changes will more negatively affect freshwater aquatic communities compared to chronic exposure to salinization where the communities have had time to adapt or turnover resulting in recovered biogeochemical functions.Competing Interest StatementThe authors have declared no competing interest.