PT - JOURNAL ARTICLE AU - Garceau, Caroline AU - Marsault, Justine AU - Robinson, Mike J F AU - Samaha, Anne-Noël TI - Metabotropic group II glutamate receptors mediate cue-triggered increases in incentive motivation for reward AID - 10.1101/2021.09.14.460377 DP - 2021 Jan 01 TA - bioRxiv PG - 2021.09.14.460377 4099 - http://biorxiv.org/content/early/2021/09/17/2021.09.14.460377.short 4100 - http://biorxiv.org/content/early/2021/09/17/2021.09.14.460377.full AB - Rationale Reward-associated cues can acquire incentive motivational properties and invigorate reward-seeking actions via Pavlovian-to-instrumental transfer (PIT). Glutamatergic neurotransmission mediates the appetitive effects of reward-associated cues. We characterized the expression of PIT and its mediation by metabotropic group II glutamate (mGlu2/3) receptor activity in female and male rats.Objectives Across the sexes, we used PIT procedures to determine i) cue-triggered increases in incentive motivation for water reward (Experiment 1), ii) the respective influences of the mGlu2/3 receptor agonist LY379268 and reward devaluation by satiation on this effect (Experiment 2).Methods Water-restricted male and female Sprague-Dawley rats learned to lever press for water. Separately, they learned that one of two auditory stimuli predicts free water (CS+ vs CS-). On PIT test days, the CS+ and CS- were presented non-contingently, and we measured effects on lever pressing under extinction (no water). In Experiment 1, we characterized PIT across the sexes. In Experiment 2, we measured PIT after systemic LY379268 administration (0, 0.3 and 1 mg/kg), and water satiation, respectively.Results Female and male rats showed similar PIT, with CS+ but not CS- presentations potentiating water-seeking behaviour. LY379268 (1 mg/kg) attenuated CS+ evoked increases in both water-associated lever pressing and conditioned approach to the water port. Reward devaluation attenuated both water-seeking and CS+ evoked conditioned approach behaviour.Conclusions The sexes show similar cue-triggered increases in reward ‘wanting’, and water devaluation suppresses both water seeking and cue-triggered anticipation of water reward. Finally, across the sexes, mGlu2/3 receptor activity mediates cue-triggered increases in reward ‘wanting’.Competing Interest StatementThe authors have declared no competing interest.