RT Journal Article
SR Electronic
T1 A simple method for in situ, multiplexed measurement of RNA degradation by flow cytometry
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.09.17.460772
DO 10.1101/2021.09.17.460772
A1 Jayan Rammohan
A1 Nina Alperovich
A1 Bin Shao
A1 David Ross
YR 2021
UL http://biorxiv.org/content/early/2021/09/17/2021.09.17.460772.abstract
AB RNA degradation plays a major role in cellular function, but current methods for measuring RNA degradation require RNA purification or are low throughput. Here we show how a flow-FISH assay can be used for high-throughput, in situ measurement of RNA degradation without RNA purification. We demonstrate how this approach can be used to simultaneously measure RNA degradation rates of different RNA sequences in a single assay and explore how the assay can be used to examine the effect of cellular context on RNA degradation rates. This assay will be generally useful to quantitatively measure how natural and engineered biological function depends on RNA half-life.Competing Interest StatementThe authors have declared no competing interest.