PT  - JOURNAL ARTICLE
AU  - Danielle G. May
AU  - Laura Martin-Sancho
AU  - Valesca Anschau
AU  - Sophie Liu
AU  - Rachel J. Chrisopulos
AU  - Kelsey L. Scott
AU  - Charles T. Halfmann
AU  - Ramon Díaz Peña
AU  - Dexter Pratt
AU  - Alexandre R. Campos
AU  - Kyle J. Roux
TI  - A BioID-derived proximity interactome for SARS-CoV-2 proteins
AID  - 10.1101/2021.09.17.460814
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.09.17.460814
4099  - http://biorxiv.org/content/early/2021/09/21/2021.09.17.460814.short
4100  - http://biorxiv.org/content/early/2021/09/21/2021.09.17.460814.full
AB  - The novel coronavirus SARS-CoV-2 is responsible for the ongoing COVID-19 pandemic and has caused a major health and economic burden worldwide. Understanding how SARS-CoV-2 viral proteins behave in host cells can reveal underlying mechanisms of pathogenesis and assist in development of antiviral therapies. Here we use BioID to map the SARS-CoV-2 virus-host interactome using human lung cancer derived A549 cells expressing individual SARS-CoV-2 viral proteins. Functional enrichment analyses revealed previously reported and unreported cellular pathways that are in association with SARS-CoV-2 proteins. We have also established a website to host the proteomic data to allow for public access and continued analysis of host-viral protein associations and whole-cell proteomes of cells expressing the viral-BioID fusion proteins. Collectively, these studies provide a valuable resource to potentially uncover novel SARS-CoV-2 biology and inform development of antivirals.Competing Interest StatementSanford Research has licensed BioID reagents to BioFront Technologies. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.