RT Journal Article
SR Electronic
T1 A BioID-derived proximity interactome for SARS-CoV-2 proteins
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.09.17.460814
DO 10.1101/2021.09.17.460814
A1 May, Danielle G.
A1 Martin-Sancho, Laura
A1 Anschau, Valesca
A1 Liu, Sophie
A1 Chrisopulos, Rachel J.
A1 Scott, Kelsey L.
A1 Halfmann, Charles T.
A1 Peña, Ramon Díaz
A1 Pratt, Dexter
A1 Campos, Alexandre R.
A1 Roux, Kyle J.
YR 2021
UL http://biorxiv.org/content/early/2021/09/21/2021.09.17.460814.abstract
AB The novel coronavirus SARS-CoV-2 is responsible for the ongoing COVID-19 pandemic and has caused a major health and economic burden worldwide. Understanding how SARS-CoV-2 viral proteins behave in host cells can reveal underlying mechanisms of pathogenesis and assist in development of antiviral therapies. Here we use BioID to map the SARS-CoV-2 virus-host interactome using human lung cancer derived A549 cells expressing individual SARS-CoV-2 viral proteins. Functional enrichment analyses revealed previously reported and unreported cellular pathways that are in association with SARS-CoV-2 proteins. We have also established a website to host the proteomic data to allow for public access and continued analysis of host-viral protein associations and whole-cell proteomes of cells expressing the viral-BioID fusion proteins. Collectively, these studies provide a valuable resource to potentially uncover novel SARS-CoV-2 biology and inform development of antivirals.Competing Interest StatementSanford Research has licensed BioID reagents to BioFront Technologies. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.