RT Journal Article
SR Electronic
T1 Virucidal activity of CPC-containing oral rinses against SARS-CoV-2 variants and are active in the presence of human saliva
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.08.05.455040
DO 10.1101/2021.08.05.455040
A1 Enyia R Anderson
A1 Edward I Patterson
A1 Siobhan Richards
A1 Alison Green
A1 Sayandip Mukherjee
A1 Michael Hoptroff
A1 Grant L Hughes
YR 2021
UL http://biorxiv.org/content/early/2021/09/22/2021.08.05.455040.abstract
AB The role of human saliva in aerosol-based transmission of SARS-CoV-2 has highlighted the need to understand the potential of oral hygiene products to inactivate the virus. Here we examined the efficacy of mouthwashes containing cetylpyridinium chloride (CPC) or chlorhexidine (CHX) in inactivating SARS-CoV-2. After 30 seconds contact under standard aqueous conditions CPC mouthwashes achieved a ≥4.0log10 PFU/mL reduction in SARS-CoV-2 (USA-WA1/2020) titres whereas comparable products containing CHX achieved <2.0log10 PFU/mL reduction. Further testing with CPC mouthwashes demonstrated efficacy against multiple SARS-CoV-2 variants, with inactivation below the limit of detection observed against the Alpha (B.1.1.7), Beta (B.1.351) and Gamma (P.1) variants. Virucidal efficacy of CPC mouthwash was also observed in the presence of human saliva with the product delivering ≥4.0log10 PFU/mL reduction in SARS-CoV-2 titres after 30 seconds providing additional evidence for the virucidal efficacy of CPC mouthwashes under simulated physiological conditions. Together these data suggest CPC-based mouthwashes are effective at inactivating SARS-CoV-2 and further supports the use of mouthwash to mitigate the risk of transmission during dentistry procedures.Competing Interest StatementAG, SM, and MH are employees of Unilever. Unilever funded this study.