PT  - JOURNAL ARTICLE
AU  - Huijie Feng
AU  - Yukun Yuan
AU  - Michael R. Williams
AU  - Alex J. Roy
AU  - Jeffery Leipprandt
AU  - Richard R. Neubig
TI  - Mice With Monoallelic <em>GNAO1</em> Loss Exhibit Reduced Inhibitory Synaptic Input To Cerebellar Purkinje Cells
AID  - 10.1101/2021.09.23.461583
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.09.23.461583
4099  - http://biorxiv.org/content/early/2021/09/24/2021.09.23.461583.short
4100  - http://biorxiv.org/content/early/2021/09/24/2021.09.23.461583.full
AB  - GNAO1 encodes Gαo, a heterotrimeric G protein alpha subunit in the Gi/o family. In this report, we used a Gnao1 mouse model “G203R” previously described as a “gain-of-function” Gnao1 mutant with movement abnormalities and enhanced seizure susceptibility. Here, we report an unexpected second mutation resulting in a loss-of-function Gαo protein and describe alterations in central synaptic transmission.Whole cell patch clamp recordings from Purkinje cells (PCs) in acute cerebellar slices from Gnao1 mutant mice showed significantly lower frequencies of spontaneous and miniature inhibitory postsynaptic currents (sIPSCs and mIPSCs) compared to WT mice. There was no significant change in sEPSCs or mEPSCs. Whereas mIPSC frequency was reduced, mIPSC amplitudes were not affected, suggesting a presynaptic mechanism of action. A modest decrease in the number of molecular layer interneurons was insufficient to explain the magnitude of IPSC suppression. Paradoxically, Gi/o inhibitors (pertussis toxin), enhanced the mutant-suppressed mIPSC frequency and eliminated the difference between WT and Gnao1 mice. While GABAB receptor regulates mIPSCs, neither agonists nor antagonists of this receptor altered function in the mutant mouse PCs. This study is the first electrophysiological investigation of the role of Gi/o protein in cerebellar synaptic transmission using an animal model with a loss-of-function Gi/o protein.Significance Statement This is the first report on the electrophysiological mechanisms of a movement disorder animal model with monoallelic Gnao1 loss. This study illustrates the role of Gαo protein in regulating GABA release in mouse cerebellum. This study could also facilitate the discovery of new drugs or drug repurposing for GNAO1-associated disorders. Moreover, since GNAO1 shares pathways with other genes related to movement disorders, developing drugs for the treatment of GNAO1-associated movement disorders could further the pharmacological intervention for other monogenic movement disorders.Competing Interest StatementThe authors have declared no competing interest.