TY - JOUR T1 - The DarTG toxin-antitoxin system provides phage defense by ADP-ribosylating viral DNA JF - bioRxiv DO - 10.1101/2021.09.27.462013 SP - 2021.09.27.462013 AU - Michele LeRoux AU - Sriram Srikant AU - Megan H. Littlehale AU - Gabriella Teodoro AU - Shany Doron AU - Mohsen Badiee AU - Anthony K. L. Leung AU - Rotem Sorek AU - Michael T. Laub Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/09/27/2021.09.27.462013.abstract N2 - Toxin-antitoxin (TA) systems are broadly distributed, yet poorly conserved, genetic elements whose biological functions are unclear and controversial. Some TA systems may provide bacteria with immunity to infection by their ubiquitous viral predators, the bacteriophage. To identify TA systems that protect E. coli MG1655 against phage, we searched for those frequently encoded near known phage defense genes in bacterial genomes. Two of the systems tested provide strong protection against phage infection and are homologs of DarTG, a recently discovered family of TA systems whose biological functions and natural activating conditions were unclear. We demonstrate that phage infection triggers the release of DarT toxin, a DNA ADP-ribosyltransferase, to modify viral DNA and prevent replication, thereby blocking the production of mature virions. Phages can evolve to overcome DarTG defense either through mutations to their DNA polymerase or to an anti-DarT factor, gp61.2, encoded by many T-even phages. Collectively, our results indicate that phage defense may be a common function for TA systems and reveal the mechanism by which DarTG systems inhibit phage infection.Competing Interest StatementThe authors have declared no competing interest. ER -