TY - JOUR T1 - <em>Streptococcus pneumoniae</em> rapidly translocates from the nasopharynx through the cribriform plate to invade and inflame the dura JF - bioRxiv DO - 10.1101/2021.09.28.462246 SP - 2021.09.28.462246 AU - Teerawit Audshasai AU - Jonathan A. Coles AU - Stavros Panagiotou AU - Shadia Khandaker AU - Hannah E. Scales AU - Morten Kjos AU - Murielle Baltazar AU - Julie Vignau AU - James M. Brewer AU - Aras Kadioglu AU - Marie Yang Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/09/29/2021.09.28.462246.abstract N2 - The entry routes and translocation mechanisms of bacterial pathogens into the central nervous system remain obscure. We report here that Streptococcus pneumoniae (Sp) or polystyrene microspheres, applied to the nose of a mouse, appeared in the meninges of the dorsal cortex within minutes. Recovery of viable bacteria from dissected tissue and fluorescence microscopy showed that up to at least 72h, Sp and microspheres were predominantly in the outer of the two meninges, the pachymeninx. No Sp were found in blood or cerebrospinal fluid. Evidence that this was not an artifact of the method of administration is that in mice infected by horizontal transmission, Sp were also predominantly in the meninges and absent from blood. Intravital imaging through the skull, and flow cytometry showed recruitment and activation of LysM+ cells in the dorsal pachymeninx at 5h and 10h following intranasal infection. Imaging of the cribriform plate suggested that both Sp and microspheres entered through its foramina via an inward flow of fluid connecting the nose to the pachymeninx. Our findings bring further insight into the invasion mechanisms of bacterial pathogens such as Sp into the central nervous system, but are also pertinent to the delivery of drugs to the brain, and the entry of air-borne particles into the cranium.Competing Interest StatementThe authors have declared no competing interest.BHIBrain Heart InfusionCSFCerebrospinal FluidCSFECarboxyFluorescein Succinimidyl EsterCNSCentral Nervous SystemDCDendritic CellCFUColony Forming UnitGFPGreen Fluorescent ProteinFCSFetal Calf SerumIAVInfluenza A VirusLSMLaser Scanning MicroscopeN.A.Numerical AperturePBSphosphate buffered salinePFAparaformaldehydeSHGSecond Harmonic GenerationSpStreptococcus pneumoniae ER -