@article {Aid2021.09.30.462514, author = {Malika Aid and Samuel J. Vidal and Cesar Piedra-Mora and Sarah Ducat and Chi N. Chan and Stephen Bondoc and Alessandro Colarusso and Carly E. Starke and Michael Nekorchuk and Kathleen Busman-Sahay and Jacob D. Estes and Amanda J. Martinot and Dan H. Barouch}, title = {Ad26.COV2.S Prevents SARS-CoV-2 Induced Pathways of Inflammation and Thrombosis in Hamsters}, elocation-id = {2021.09.30.462514}, year = {2021}, doi = {10.1101/2021.09.30.462514}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Syrian golden hamsters exhibit features of severe disease after SARS-CoV-2 challenge and are therefore useful models of COVID-19 pathogenesis and prevention with vaccines. Recent studies have shown that SARS-CoV-2 infection stimulates type I interferon, myeloid, and inflammatory signatures similar to human disease, and that weight loss can be prevented with vaccines. However, the impact of vaccination on transcriptional programs associated with COVID-19 pathogenesis and protective adaptive immune responses is unknown. Here we show that SARS-CoV-2 challenge in hamsters stimulates antiviral, myeloid, and inflammatory programs as well as signatures of complement and thrombosis associated with human COVID-19. Notably, single dose immunization with Ad26.COV2.S, an adenovirus serotype 26 vector (Ad26)-based vaccine expressing a stabilized SARS-CoV-2 spike protein, prevents the upregulation of these pathways such that the gene expression profiles of vaccinated hamsters are comparable to uninfected animals. Finally, we show that Ad26.COV2.S vaccination induces T and B cell signatures that correlate with binding and neutralizing antibody responses. These data provide further insights into the mechanisms of Ad26.COV2.S based protection against severe COVID-19 in hamsters.Author Summary In this study, we show that vaccination with Ad26.COV2.S protected SARS-CoV-2 challenged hamsters from developing severe COVID-19 disease by attenuating excessive proinflammatory responses, such as IL-6 and IL-1, macrophages and neutrophils signaling. Ad26 vaccination also prevented the upregulation of pathways associated with thrombosis such coagulation and clotting cascades associated with infection, and the transcriptomic profiles of vaccinated animals were largely comparable to control uninfected hamsters. In contrast, SARS-CoV-2 challenged unvaccinated hamsters showed significant increase of these proinflammatory and prothrombotic pathways and significant weight loss compared to vaccinated hamsters.Competing Interest StatementD.H.B. is a co-inventor on COVID-19 vaccine related patents.}, URL = {https://www.biorxiv.org/content/early/2021/09/30/2021.09.30.462514}, eprint = {https://www.biorxiv.org/content/early/2021/09/30/2021.09.30.462514.full.pdf}, journal = {bioRxiv} }