RT Journal Article
SR Electronic
T1 Engineering species-like barriers to sexual reproduction
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 079095
DO 10.1101/079095
A1 Maciej Maselko
A1 Stephen C. Heinsch
A1 Jeremy Chacón
A1 William Harcombe
A1 Michael J. Smanski
YR 2016
UL http://biorxiv.org/content/early/2016/10/04/079095.1.abstract
AB We introduce a novel approach to engineer a genetic barrier to sexual reproduction between otherwise compatible populations. Programmable transcription factors drive lethal gene expression in hybrid offspring following undesired mating events. As a proof of concept, we target the ACT1 promoter of the model organism Saccharomyces cerevisiae using a dCas9-based transcriptional activator. Lethal over-expression of actin results from mating this engineered strain with a strain containing the wild-type ACT1 promoter.