RT Journal Article
SR Electronic
T1 Two-color super-resolution localization microscopy via joint encoding of emitter location and color
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.10.03.462920
DO 10.1101/2021.10.03.462920
A1 Yujie Wang
A1 Weibing Kuang
A1 Mingtao Shang
A1 Zhen-Li Huang
YR 2021
UL http://biorxiv.org/content/early/2021/10/03/2021.10.03.462920.abstract
AB Multi-color super-resolution localization microscopy (SRLM) provides great opportunities for studying the structural and functional details of biological samples. However, current multi-color SRLM methods either suffer from medium to high crosstalk, or require a dedicated optical system and a complicated image analysis procedure. To address these problems, here we propose a completely different method to realize multi-color SRLM. This method is built upon a customized RGBW camera with a repeated pattern of filtered (Red, Green, Blue and Near-infrared) and unfiltered (White) pixels. With a new insight that RGBW camera is advantageous for color recognition instead of color reproduction, we developed a joint encoding scheme of emitter location and color. By combing this RGBW camera with the joint encoding scheme and a simple optical set-up, we demonstrated two-color SRLM with ∼20 nm resolution and < 2% crosstalk (which is comparable to the best reported values). This study significantly reduces the complexity of two-color SRLM (and potentially multi-color SRLM), and thus offers good opportunities for general biomedical research laboratories to use multi-color SRLM, which is currently mastered only by well-trained researchers.Competing Interest StatementChinese patent (No. 201911365462.2).