RT Journal Article
SR Electronic
T1 SCORPIUS improves trajectory inference and identifies novel modules in dendritic cell development
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 079509
DO 10.1101/079509
A1 Robrecht Cannoodt
A1 Wouter Saelens
A1 Dorine Sichien
A1 Simon Tavernier
A1 Sophie Janssens
A1 Martin Guilliams
A1 Bart Lambrecht
A1 Katleen De Preter
A1 Yvan Saeys
YR 2016
UL http://biorxiv.org/content/early/2016/10/06/079509.abstract
AB Recent advances in RNA sequencing enable the generation of genome-wide expression data at the single-cell level, opening up new avenues for transcriptomics and systems biology. A new application of single-cell whole-transcriptomics is the unbiased ordering of cells according to their progression along a dynamic process of interest. We introduce SCORPIUS, a method which can effectively reconstruct an ordering of individual cells without any prior information about the dynamic process. Comprehensive evaluation using ten scRNA-seq datasets shows that SCORPIUS consistently outperforms state-of-the-art techniques. We used SCORPIUS to generate novel hypotheses regarding dendritic cell development, which were subsequently validated in vivo. This work enables data-driven investigation and characterization of dynamic processes and lays the foundation for objective benchmarking of future trajectory inference methods.