PT  - JOURNAL ARTICLE
AU  - Sheng-Yi Wu
AU  - Yurong Wen
AU  - Nelson Bernard Calixte Serre
AU  - Cathrine Charlotte Heiede Laursen
AU  - Andrea Grostøl Dietz
AU  - Brian R. Taylor
AU  - Abhi Aggarwal
AU  - Vladimir Rancic
AU  - Michael Becker
AU  - Klaus Ballanyi
AU  - Kaspar Podgorski
AU  - Hajime Hirase
AU  - Maiken Nedergaard
AU  - Matyáš Fendrych
AU  - M. Joanne Lemieux
AU  - Daniel F. Eberl
AU  - Alan R. Kay
AU  - Robert E. Campbell
AU  - Yi Shen
TI  - A sensitive and specific genetically encodable biosensor for potassium ions
AID  - 10.1101/2021.10.07.463410
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.10.07.463410
4099  - http://biorxiv.org/content/early/2021/10/09/2021.10.07.463410.short
4100  - http://biorxiv.org/content/early/2021/10/09/2021.10.07.463410.full
AB  - Potassium ions (K+) play a critical role as an essential electrolyte in all biological systems. Here we report the crystal structure-guided optimization and directed evolution of an improved genetically encoded fluorescent K+ biosensor, GINKO2. GINKO2 is highly sensitive and specific for K+ and enables in vivo detection of K+ dynamics in multiple species.Competing Interest StatementThe authors have declared no competing interest.