TY - JOUR T1 - Exploiting noise, non-linearity, and feedback for differential control of multiple synthetic cells with a single optogenetic input JF - bioRxiv DO - 10.1101/2021.05.11.443599 SP - 2021.05.11.443599 AU - Michael P May AU - Brian Munsky Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/10/09/2021.05.11.443599.abstract N2 - Synthetic biology seeks to develop modular bio-circuits that combine to produce complex, controllable behaviors. These designs are often subject to noisy fluctuations and uncertainties, and most modern synthetic biology design processes have focused to create robust components to mitigate the noise of gene expression and reduce the heterogeneity of single-cell responses. However, deeper understanding of noise can achieve control goals that would otherwise be impossible. We explore how an “Optogenetic Maxwell Demon” could selectively amplify noise to control multiple cells using single-input-multiple-output (SIMO) feedback. Using data-constrained stochastic model simulations and theory, we show how an appropriately selected stochastic SIMO controller can drive multiple different cells to different user-specified configurations irrespective of initial condition. We explore how controllability depends on cells’ regulatory structures, the amount of information available to the controller, and the accuracy of the model used. Our results suggest that gene regulation noise, when combined with optogenetic feedback and non-linear biochemical auto-regulation, can achieve synergy to enable precise control of complex stochastic processes.Competing Interest StatementThe authors have declared no competing interest. ER -