PT  - JOURNAL ARTICLE
AU  - Robrecht Cannoodt
AU  - Wouter Saelens
AU  - Dorine Sichien
AU  - Simon Tavernier
AU  - Sophie Janssens
AU  - Martin Guilliams
AU  - Bart Lambrecht
AU  - Katleen De Preter
AU  - Yvan Saeys
TI  - SCORPIUS improves trajectory inference and identifies novel modules in dendritic cell development
AID  - 10.1101/079509
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 079509
4099  - http://biorxiv.org/content/early/2016/10/07/079509.short
4100  - http://biorxiv.org/content/early/2016/10/07/079509.full
AB  - Recent advances in RNA sequencing enable the generation of genome-wide expression data at the single-cell level, opening up new avenues for transcriptomics and systems biology. A new application of single-cell whole-transcriptomics is the unbiased ordering of cells according to their progression along a dynamic process of interest. We introduce SCORPIUS, a method which can effectively reconstruct an ordering of individual cells without any prior information about the dynamic process. Comprehensive evaluation using ten scRNA-seq datasets shows that SCORPIUS consistently outperforms state-of-the-art techniques. We used SCORPIUS to generate novel hypotheses regarding dendritic cell development, which were subsequently validated in vivo. This work enables data-driven investigation and characterization of dynamic processes and lays the foundation for objective benchmarking of future trajectory inference methods.