PT - JOURNAL ARTICLE AU - Anton Suvorov AU - Bernard Y. Kim AU - Jeremy Wang AU - Ellie E. Armstrong AU - David Peede AU - Emmanuel R. R. D’Agostino AU - Donald K. Price AU - Peter Wadell AU - Michael Lang AU - Virginie Courtier-Orgogozo AU - Jean R. David AU - Dmitri Petrov AU - Daniel R. Matute AU - Daniel R. Schrider AU - Aaron A. Comeault TI - Widespread introgression across a phylogeny of 155 <em>Drosophila</em> genomes AID - 10.1101/2020.12.14.422758 DP - 2021 Jan 01 TA - bioRxiv PG - 2020.12.14.422758 4099 - http://biorxiv.org/content/early/2021/10/10/2020.12.14.422758.short 4100 - http://biorxiv.org/content/early/2021/10/10/2020.12.14.422758.full AB - Genome-scale sequence data have invigorated the study of hybridization and introgression, particularly in animals. However, outside of a few notable cases, we lack systematic tests for introgression at a larger phylogenetic scale across entire clades. Here we leverage 155 genome assemblies, from 149 species, to generate a fossil-calibrated phylogeny and conduct multilocus tests for introgression across nine monophyletic radiations within the genus Drosophila. Using complementary phylogenomic approaches, we identify widespread introgression across the evolutionary history of Drosophila. Mapping gene-tree discordance onto the phylogeny revealed that both ancient and recent introgression has occurred across most of the nine clades that we examined. Our results provide the first evidence of introgression occurring across the evolutionary history of Drosophila and highlight the need to continue to study the evolutionary consequences of hybridization and introgression in this genus and across the Tree of Life.Competing Interest StatementThe authors have declared no competing interest.