PT - JOURNAL ARTICLE AU - R.J. Sepela AU - R.G. Stewart AU - L.A. Valencia AU - P. Thapa AU - Z. Wang AU - B.E. Cohen AU - J.T. Sack TI - The AMIGO1 adhesion protein activates Kv2.1 voltage sensors AID - 10.1101/2021.06.20.448455 DP - 2021 Jan 01 TA - bioRxiv PG - 2021.06.20.448455 4099 - http://biorxiv.org/content/early/2021/10/11/2021.06.20.448455.short 4100 - http://biorxiv.org/content/early/2021/10/11/2021.06.20.448455.full AB - Kv2 voltage-gated potassium channels are modulated by AMIGO neuronal adhesion proteins. Here, we identify steps in the conductance activation pathway of Kv2.1 channels that are modulated by AMIGO1 using voltage clamp recordings and spectroscopy of heterologously expressed Kv2.1 and AMIGO1 in mammalian cell lines. AMIGO1 speeds early voltage sensor movements and shifts the gating charge–voltage relationship to more negative voltages. The gating charge–voltage relationship indicates that AMIGO1 exerts a larger energetic effect on voltage sensor movement than apparent from the midpoint of the conductance–voltage relationship. When voltage sensors are detained at rest by voltage sensor toxins, AMIGO1 has a greater impact on the conductance–voltage relationship. Fluorescence measurements from voltage sensor toxins bound to Kv2.1 indicate that with AMIGO1, the voltage sensors enter their earliest resting conformation, yet this conformation is less stable upon voltage stimulation. We conclude that AMIGO1 modulates the Kv2.1 conductance activation pathway by destabilizing the earliest resting state of the voltage sensors.Statement of Significance Kv2 potassium channels activate a potassium conductance that shapes neuronal action potentials. The AMIGO family of adhesion proteins modulate activation of Kv2 conductances, yet, which activation steps are modified is unknown. This study finds that AMIGO1 destabilizes the earliest resting conformation of the Kv2.1 voltage sensors to promote activation of channel conductance.Competing Interest StatementThe authors have declared no competing interest.