RT Journal Article
SR Electronic
T1 Intercellular alignment of apical-basal polarity coordinates tissue homeostasis and growth
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.10.11.463906
DO 10.1101/2021.10.11.463906
A1 Jinghua Gui
A1 Yunxian Huang
A1 Satu-Marja Myllymäki
A1 Marja Mikkola
A1 Osamu Shimmi
YR 2021
UL http://biorxiv.org/content/early/2021/10/12/2021.10.11.463906.abstract
AB Maintaining apicobasal polarity (ABP) is crucial for epithelial integrity and homeostasis during tissue development. Although recent studies have greatly advanced our understanding of intracellular mechanisms underlying ABP establishment, it remains largely unknown how the ABP is regulated at the tissue level. Here, we address intercellular mechanisms coordinating ABP using the Drosophila wing imaginal disc. By studying Scribble, a key ABP determinant, we show that ABP is regulated through intercellular alignment, which takes place either progressively or regressively in a context-dependent manner. Cells expressing wild type scribble progressively restore ABP in scribble hypomorphic mutant cells. In contrast, cells with conditional scribble knockdown instigate the regressive loss of polarity in abutting wild type cells. Our data reveal that genetic and physical interactions between Scribble, Septate junction complex and α-Catenin appear to be key for sustaining intercellular network of ABP. Taken together, our findings indicate that the intercellular relay of the status of ABP contributes to the robustness of polarity across the tissue.Competing Interest StatementThe authors have declared no competing interest.