PT  - JOURNAL ARTICLE
AU  - Markus Mund
AU  - Aline Tschanz
AU  - Yu-Le Wu
AU  - Felix Frey
AU  - Johanna L. Mehl
AU  - Marko Kaksonen
AU  - Ori Avinoam
AU  - Ulrich S. Schwarz
AU  - Jonas Ries
TI  - Superresolution microscopy reveals partial preassembly and subsequent bending of the clathrin coat during endocytosis
AID  - 10.1101/2021.10.12.463947
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.10.12.463947
4099  - http://biorxiv.org/content/early/2021/10/13/2021.10.12.463947.short
4100  - http://biorxiv.org/content/early/2021/10/13/2021.10.12.463947.full
AB  - Eukaryotic cells use clathrin-mediated endocytosis to take up a large range of extracellular cargos. During endocytosis, a clathrin coat forms on the plasma membrane, but it remains controversial when and how it is remodeled into a spherical vesicle.Here, we use 3D superresolution microscopy to determine the precise geometry of the clathrin coat at endocytic sites. Through pseudo-temporal sorting, we determine the average trajectory of clathrin remodeling during endocytosis and find that clathrin coats assemble first on flat membranes to ≈50% of the coat area, before they become rapidly and continuously bent. We introduce a mathematical model that assumes a positive feedback for curvature generation of the clathrin coat. The cooperative curvature model agrees excellently with experimental data in three cell lines, and likely describes a general pathway for clathrin coat remodeling during endocytosis.Competing Interest StatementThe authors have declared no competing interest.