RT Journal Article
SR Electronic
T1 Long-range migration of centrioles to the apical surface of the olfactory epithelium
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.10.12.464082
DO 10.1101/2021.10.12.464082
A1 Ching, Kaitlin
A1 Wang, Jennifer T.
A1 Stearns, Tim
YR 2021
UL http://biorxiv.org/content/early/2021/10/13/2021.10.12.464082.abstract
AB Olfactory sensory neurons (OSNs) in vertebrates detect odorants using multiple cilia, which protrude from the end of the dendrite and require centrioles for their formation. In mouse olfactory epithelium, the centrioles originate in progenitor cells near the basal lamina, often 50 to 100 μm from the apical surface. It is unknown how centrioles traverse this distance or mature to form cilia. Using high-resolution expansion microscopy, we found that centrioles migrate together, with multiple centrioles per group and multiple groups per OSN, during dendrite outgrowth. Centrioles were found by live imaging to migrate slowly, with a maximum rate of 0.18 μm/min. Centrioles in migrating groups were associated with microtubule nucleation factors, but acquired rootletin and appendages only in mature OSNs. The parental centriole had preexisting appendages, formed a single cilium prior to other centrioles, and retained its unique appendage configuration in the mature OSN. We developed an air-liquid interface explant culture system for OSNs and used it to show that centriole migration can be perturbed ex vivo by stabilizing microtubules. We consider these results in the context of a comprehensive model for centriole formation, migration, and maturation in this important sensory cell type.Competing Interest StatementThe authors have declared no competing interest.