TY - JOUR T1 - Development of a metatranscriptomic analysis method for multiple intestinal sites and its application to the common marmoset intestine JF - bioRxiv DO - 10.1101/2021.10.12.464166 SP - 2021.10.12.464166 AU - Mika Uehara AU - Takashi Inoue AU - Minori Kominato AU - Sumitaka Hase AU - Erika Sasaki AU - Atsushi Toyoda AU - Yasubumi Sakakibara Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/10/13/2021.10.12.464166.abstract N2 - Background The intestinal microbiome is closely related to host health, and metatranscriptomic analysis can assess the functional activity of microbiomes by quantifying the bacterial gene expression level, which helps to elucidate the interaction between the microbiome and the environment. However, functional changes in the microbiome along the host intestinal tract remain unknown, and previous analytical methods have limitations, such as potentially overlooking unknown genes due to dependence on existing databases and being unable to take full advantage of metatranscriptome to reveal the functional change among multiple environments.Result To close these gaps, we develop a novel method that integrates metagenome and metatranscriptome to analyze the functional activity of microbiomes between intestinal sites. This method reconstructs a reference metagenomic sequence across multiple intestinal sites, allowing the gene expression levels of microbiome including unknown bacterial genes to be compared between multiple sites. As a result of applying this method to metatranscriptomic analysis in the intestinal tract of common marmoset, the reconstructed metagenome covered most of the expressed genes and it revealed that the changes in bacterial gene expressions among the caecum, transverse colon, and faeces were more dynamic and sensitive to environmental shifts than its abundance. In typical, the coenzyme synthesis gene and antibacterial resistance gene were more highly expressed in the caecum and transverse colon than in faeces, while there was no significant change in abundance of these genes.Conclusion Our findings demonstrate that an analytical method that integrates metagenome and metatranscriptome in multiple intestinal sites captures functional changes in the microbiomes at the gene resolution level.Competing Interest StatementThe authors have declared no competing interest. ER -