PT  - JOURNAL ARTICLE
AU  - Elisa Violeta Bertini
AU  - Mariela Analía Torres
AU  - Thibaut Léger
AU  - Camille Garcia
AU  - Kar-Wai Hong
AU  - Teik Min Chong
AU  - Lucía I. Castellanos de Figueroa
AU  - Kok-Gan Chan
AU  - Yves Dessaux
AU  - Jean-Michel Camadro
AU  - Carlos Gabriel Nieto-Peñalver
TI  - Insight in the quorum sensing-driven lifestyle of the non-pathogenic <em>Agrobacterium tumefaciens</em> 6N2 and the interactions with the yeast <em>Meyerozyma guilliermondii</em>
AID  - 10.1101/2021.10.17.464673
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.10.17.464673
4099  - http://biorxiv.org/content/early/2021/10/17/2021.10.17.464673.short
4100  - http://biorxiv.org/content/early/2021/10/17/2021.10.17.464673.full
AB  - Agrobacterium tumefaciens is considered a prominent phytopathogen, though most isolates are nonpathogenic. Agrobacteria can inhabit plant tissues interacting with other microorganisms. Yeasts are likewise part of these communities. We analyzed the quorum sensing (QS) systems of A. tumefaciens strain 6N2, and its relevance for the interaction with the yeast Meyerozyma guilliermondii, both sugarcane endophytes. We show that strain 6N2 is nonpathogenic, produces OHC8-HSL, OHC10-HSL, OC12-HSL and OHC12-HSL as QS signals, and possesses a complex QS architecture, with one truncated, two complete systems, and three additional QS-signal receptors. A proteomic approach showed differences in QS-regulated proteins between pure (64 proteins) and dual (33 proteins) cultures. Seven proteins were consistently regulated by quorum sensing in pure and dual cultures. M. guilliermondii proteins influenced by QS activity were also evaluated. Several up- and down- regulated proteins differed depending on the bacterial QS. These results show the importance of the QS regulation in the bacteria-yeast interactions.Highlights The avirulent A. tumefaciens 6N2 has two replicons and a complex QS architectureThe profile of QS-regulated proteins is modified in dual cultures with Pa. laurentiiThe bacterial QS activity alters the proteome of the yeast Pa. laurentiiCompeting Interest StatementThe authors have declared no competing interest.