RT Journal Article
SR Electronic
T1 A Mediterranean-like fat blend protects against the development of severe colitis in the mucin-2 deficient murine model
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.09.27.462042
DO 10.1101/2021.09.27.462042
A1 Natasha Haskey
A1 Jiayu Ye
A1 Mehrbod Estaki
A1 Andrea A. Verdugo Meza
A1 Jacqueline A. Barnett
A1 Mitra Yousefi
A1 Blake W. Birnie
A1 Samantha Gruenheid
A1 Sanjoy Ghosh
A1 Deanna L. Gibson
YR 2021
UL http://biorxiv.org/content/early/2021/10/17/2021.09.27.462042.abstract
AB The Mediterranean diet (MD) is a health-promoting, moderate fat diet (~ 40% energy). It is not known if the blend of fats found in the MD contribute to the beneficial protective effects. We compared the MD fat blend (high monounsaturated, 2:1 n-6:n-3 polyunsaturated and moderate saturated fat) to isocaloric diets composed with corn oil (CO, n-6 polyunsaturated-rich), olive oil (high monounsaturated-rich) or milk fat (MF, saturated-rich) on spontaneous colitis development in Muc2−/− mice. The MD resulted in lower clinical and histopathological scores, and induced tolerogenic CD103+CD11b+ dendritic, Th22 and IL-17+IL-22+ cells important for intestinal barrier repair. MD also reduced attendant insulin resistance and a shift to a higher health-promoting gut microbes including Lactobacillus B. animalis and Muribaculaceae, whereas CO showed higher prevalence of mucin-degraders (Akkermansia muciniphila) and colitis promoters (Enterobacteriaceae). Our findings suggest that the MD fat blend could be recommended as a maintenance diet for colitis.Competing Interest StatementThe authors have declared no competing interest.