PT  - JOURNAL ARTICLE
AU  - Irene Costantini
AU  - Leah Morgan
AU  - Jiarui Yang
AU  - Yael Balbastre
AU  - Divya Varadarajan
AU  - Luca Pesce
AU  - Marina Scardigli
AU  - Giacomo Mazzamuto
AU  - Vladislav Gavryusev
AU  - Filippo Maria Castelli
AU  - Matteo Roffilli
AU  - Ludovico Silvestri
AU  - Jessie Laffey
AU  - Sophia Raia
AU  - Merina Varghese
AU  - Bridget Wicinski
AU  - Shuaibin Chang
AU  - Anderson Chen I-Chun
AU  - Hui Wang
AU  - Devani Cordero
AU  - Matthew Vera
AU  - Jackson Nolan
AU  - Kim Nestor
AU  - Jocelyn Mora
AU  - Juan Eugenio Iglesias
AU  - Erendira Garcia Pallares
AU  - Kathryn Evancic
AU  - Jean Augustinack
AU  - Morgan Fogarty
AU  - Adrian V. Dalca
AU  - Matthew Frosch
AU  - Caroline Magnain
AU  - Robert Frost
AU  - Andre van der Kouwe
AU  - Shih-Chi Chen
AU  - David A. Boas
AU  - Francesco Saverio Pavone
AU  - Bruce Fischl
AU  - Patrick R. Hof
TI  - A multimodal imaging and analysis pipeline for creating a cellular census of the human cerebral cortex
AID  - 10.1101/2021.10.20.464979
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.10.20.464979
4099  - http://biorxiv.org/content/early/2021/10/21/2021.10.20.464979.short
4100  - http://biorxiv.org/content/early/2021/10/21/2021.10.20.464979.full
AB  - Cells are not uniformly distributed in the human cerebral cortex. Rather, they are arranged in a regional and laminar fashion that span a range of scales. Here we demonstrate an innovative imaging and analysis pipeline to construct a reliable cell census across the human cerebral cortex. Magnetic resonance imaging (MRI) is used to establish a macroscopic reference coordinate system of laminar and cytoarchitectural boundaries. Cell counting is obtained with both traditional immunohistochemistry, to provide a stereological gold-standard, and with a custom-made inverted confocal light-sheet fluorescence microscope (LSFM) for 3D imaging at cellular resolution. Finally, mesoscale optical coherence tomography (OCT) enables the registration of the distorted histological cell typing obtained with LSFM to the MRI-based atlas coordinate system.Competing Interest StatementBF has a financial interest in CorticoMetrics, a company whose medical pursuits focus on brain imaging and measurement technologies. BF's interests were reviewed and are managed by Massachusetts General Hospital and Partners HealthCare in accordance with their conflict of interest policies.