RT Journal Article
SR Electronic
T1 Vision-dependent specification of cell types and function in the developing cortex
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.08.10.455824
DO 10.1101/2021.08.10.455824
A1 Sarah Cheng
A1 Salwan Butrus
A1 Liming Tan
A1 Vincent Xu
A1 Srikant Sagireddy
A1 Joshua T. Trachtenberg
A1 Karthik Shekhar
A1 S. Lawrence Zipursky
YR 2021
UL http://biorxiv.org/content/early/2021/10/21/2021.08.10.455824.abstract
AB The role of postnatal experience in sculpting cortical circuitry, while long appreciated, is poorly understood at the level of cell types. We explore this in the mouse primary visual cortex (V1) using single-nucleus RNA-sequencing, visual deprivation, genetics, and functional imaging. We find that vision selectively drives the specification of glutamatergic cell types in upper layers (L) (L2/3/4), while deeper-layer glutamatergic, GABAergic, and non-neuronal cell types are established prior to eye opening. L2/3 cell types form an experience-dependent spatial continuum defined by the graded expression of ∼200 genes, including regulators of cell adhesion and synapse formation. Vision-dependent regulation of one of these genes, encoding the inhibitory synaptic cell adhesion molecule IGSF9b, is required for the normal development of binocular responses in L2/3. In summary, vision preferentially regulates the development of upper-layer glutamatergic cell types through the regulation of cell type-specific gene expression programs.Competing Interest StatementThe authors have declared no competing interest.