PT  - JOURNAL ARTICLE
AU  - Yijie Geng
AU  - Randall T. Peterson
TI  - Social behavioral profiling by unsupervised deep learning reveals a stimulative effect of dopamine D3 agonists on zebrafish sociality
AID  - 10.1101/2021.09.24.461752
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.09.24.461752
4099  - http://biorxiv.org/content/early/2021/10/26/2021.09.24.461752.short
4100  - http://biorxiv.org/content/early/2021/10/26/2021.09.24.461752.full
AB  - It has been a major challenge to systematically evaluate and compare how pharmacological perturbations influence social behavioral outcomes. Although some pharmacological agents are known to alter social behavior, precise description and quantification of such effects have proven difficult. The complexity of brain functions regulating sociality makes it challenging to predict drug effects on social behavior without testing in live animals, and most existing behavioral assays are low-throughput and provide only unidimensional readouts of social function. To achieve richer characterization of drug effects on sociality, we developed a scalable social behavioral assay for zebrafish named ZeChat based on unsupervised deep learning. High-dimensional and dynamic social behavioral phenotypes are automatically classified using this method. By screening a neuroactive compound library, we found that different classes of chemicals evoke distinct patterns of social behavioral fingerprints. By examining these patterns, we discovered that dopamine D3 agonists possess a social stimulative effect on zebrafish. The D3 agonists pramipexole, piribedil, and 7-hydroxy-DPAT-HBr rescued social deficits in a valproic acid-induced zebrafish autism model. The ZeChat platform provides a promising approach for dissecting the pharmacology of social behavior and discovering novel social-modulatory compounds.Competing Interest StatementThe authors have declared no competing interest.