RT Journal Article
SR Electronic
T1 Tracking neural activity from the same cells during the entire adult life of mice
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.10.29.466524
DO 10.1101/2021.10.29.466524
A1 Siyuan Zhao
A1 Xin Tang
A1 Sebastian Partarrieu
A1 Shiqi Guo
A1 Ren Liu
A1 Jaeyong Lee
A1 Zuwan Lin
A1 Jia Liu
YR 2021
UL http://biorxiv.org/content/early/2021/11/01/2021.10.29.466524.abstract
AB Recording the activity of the same neurons over the adult life of an animal is important to neuroscience research and biomedical applications. Current implantable devices cannot provide stable recording on this time scale. Here, we introduce a method to precisely implant nanoelectronics with an open, unfolded mesh structure across multiple brain regions in the mouse. The open mesh structure forms a stable interwoven structure with the neural network, preventing probe drifting and showing no immune response and neuron loss during the yearlong implantation. Using the implanted nanoelectronics, we can track single-unit action potentials from the same neurons over the entire adult life of mice. Leveraging the stable recordings, we build machine learning algorithms that enable automated spike sorting, noise rejection, stability validation, and generate pseudotime analysis, revealing aging-associated evolution of the single-neuron activities.Competing Interest StatementThe authors have declared no competing interest.