PT  - JOURNAL ARTICLE
AU  - Matochko, Wadim L.
AU  - Deiss, Frédérique
AU  - Yang, Yang
AU  - Derda, Ratmir
TI  - Reproducible Discovery of Cell-Binding Peptides “Lost” in Bulk Amplification via Emulsion Amplification in Phage Display Panning
AID  - 10.1101/2021.10.31.466683
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.10.31.466683
4099  - http://biorxiv.org/content/early/2021/11/03/2021.10.31.466683.short
4100  - http://biorxiv.org/content/early/2021/11/03/2021.10.31.466683.full
AB  - Many pharmaceutically-relevant cell surface receptors are functional only in the context of intact cells. Phage display, while being a powerful method for the discovery of ligands for purified proteins often fails to identify a diverse set of ligands to receptors on a cell membrane mosaic. To understand this deficiency, we examined growth bias in naïve phage display libraries and observed that it fundamentally changes selection outcomes: The presence of growth-biased (parasite) phage clones in a phage library is detrimental to selection and cell-based panning of such biased libraries is poised to yield ligands from within a small parasite population. Importantly, amplification of phage libraries in water-oil emulsions suppressed the amplification of parasites and steered the selection of biased phage libraries away from parasite population. Attenuation of the growth bias through the use of emulsion amplification reproducibly discovers the ligands for cell-surface receptors that cannot be identified in screen that use conventional ‘bulk’ amplification.Find Ligands in DropletsCompeting Interest StatementThe authors have declared no competing interest.