RT Journal Article
SR Electronic
T1 Minimizing spectral overlap in multicolor flow cytometry experiments
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.03.17.435861
DO 10.1101/2021.03.17.435861
A1 Lars Rønn Olsen
A1 Alfredo Benso
A1 Gianfranco Politano
A1 Mike Bogetofte Barnkob
YR 2021
UL http://biorxiv.org/content/early/2021/11/06/2021.03.17.435861.abstract
AB Selecting fluorochromes for polychromatic panels for flow cytometry is complex and time-consuming. Poorly designed panels can result in overlap between the emission spectra of the different fluorochromes, making their signals difficult to separate. While assessing all possible panels is simple to do programmatically, the combinatorial complexity of most real world problems renders brute force computation impractical. Here we present a novel complexity optimization algorithm for fast design of minimal spectral overlap fluorochrome combinations. To aid researchers in designing fluorochrome panels we implemented the algorithm in a web server, Spectracular, that also considers instrument laser configuration and allows users to define various constraints related to antibody-fluorochrome availability and marker co-expression.Competing Interest StatementThe authors have declared no competing interest.