RT Journal Article
SR Electronic
T1 Wheat inositol pyrophosphate kinase TaVIH2-3B modulates cell-wall composition for drought tolerance in Arabidopsis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 743294
DO 10.1101/743294
A1 Anuj Shukla
A1 Mandeep Kaur
A1 Swati Kanwar
A1 Gazaldeep Kaur
A1 Shivani Sharma
A1 Shubhra Ganguli
A1 Vandana Kumari
A1 Koushik Mazumder
A1 Pratima Pandey
A1 Hatem Rouached
A1 Vikas Rishi
A1 Rashna Bhandari
A1 Ajay K Pandey
YR 2021
UL http://biorxiv.org/content/early/2021/11/06/743294.abstract
AB Background Inositol pyrophosphates (PP-InsPs) are high-energy cellular molecules involved in different signalling and regulatory responses. Two distinct classes of inositol phosphate kinases responsible for the synthesis of PP-InsPs have been identified in Arabidopsis i.e. ITPKinase (inositol 1,3,4 trisphosphate 5/6 kinase) and PP-IP5Kinase (diphosphoinositol pentakisphosphate kinases). Plant PP-IP5Ks are capable of synthesizing InsP8 and were shown to control pathogenic defence and phosphate response signals. However, other roles offered by plant PP-IP5Ks, especially towards abiotic stress, remain poorly understood.Results Here, we characterized two Triticum aestivum L. (hexaploid wheat) PPIP5K homologs, TaVIH1 and TaVIH2, for their physiological functions. We demonstrated that wheat VIH proteins could utilize InsP7 as the substrate to produce InsP8, a process that requires the functional VIH-kinase domains. At the transcriptional level, both TaVIH1 and TaVIH2 are expressed in different wheat tissues, including developing grains, but show selective response to abiotic stresses during drought-mimic experiments. Overexpression of TaVIH2-3B homolog in Arabidopsis conferred tolerance to drought stress and rescued the sensitivity of Atvih2 mutants. RNAseq analysis of TaVIH2-3B transgenic lines of Arabidopsis showed a genome-wide reprogramming with remarkable effects on cell-wall biosynthesis genes with enhanced the accumulation of polysaccharides (arabinogalactan, cellulose and arabinoxylan).Conclusions Overall, this work identifies a novel function of VIH proteins, implying their roles in modulating cell-wall homeostasis genes and providing water-deficit stress tolerance. This work suggests that the plant VIH enzymes could be linked to drought tolerance and also opens up investigations to address the roles of plant VIH derived products in generating drought resistant plants.Competing Interest StatementThe authors have declared no competing interest.