PT  - JOURNAL ARTICLE
AU  - Daniela Numberger
AU  - Luca Zoccarato
AU  - Jason Woodhouse
AU  - Lars Ganzert
AU  - Sascha Sauer
AU  - Hans-Peter Grossart
AU  - Alex Greenwood
TI  - Urbanization promotes specific bacteria in freshwater microbiomes including potential pathogens
AID  - 10.1101/2020.06.26.173328
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2020.06.26.173328
4099  - http://biorxiv.org/content/early/2021/11/08/2020.06.26.173328.short
4100  - http://biorxiv.org/content/early/2021/11/08/2020.06.26.173328.full
AB  - Freshwater ecosystems are characterized by complex and highly dynamic microbial communities that are strongly structured by their local environment and biota. Growing city populations and the process of urbanization substantially alter freshwater environments. To determine the changes in freshwater microbial communities associated with urbanization, full-length 16S rRNA gene PacBio sequencing was performed from surface water and sediments from a wastewater treatment plant, urban and rural lakes in the Berlin-Brandenburg region, Northeast Germany. Water samples exhibited highly habitat specific bacterial communities with multiple genera showing clear urban signatures. We identified potentially harmful bacterial groups associated with environmental parameters specific to urban habitats such as Alistipes, Escherichia/Shigella, Rickettsia and Streptococcus. We demonstrate that urbanization alters natural microbial communities in lakes and, via simultaneous eutrophication, creates favorable conditions that promote specific bacterial genera including potential pathogens. Our findings are of global relevance highlighting a long-term health risk in urbanized waterbodies, at a time of accelerated global urbanization. The results demonstrate the urgency for undertaking mitigation measures such as targeted lake restoration projects and sustainable water management efforts.Competing Interest StatementThe authors have declared no competing interest.