TY - JOUR T1 - Urbanization promotes specific bacteria in freshwater microbiomes including potential pathogens JF - bioRxiv DO - 10.1101/2020.06.26.173328 SP - 2020.06.26.173328 AU - Daniela Numberger AU - Luca Zoccarato AU - Jason Woodhouse AU - Lars Ganzert AU - Sascha Sauer AU - Hans-Peter Grossart AU - Alex Greenwood Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/11/08/2020.06.26.173328.abstract N2 - Freshwater ecosystems are characterized by complex and highly dynamic microbial communities that are strongly structured by their local environment and biota. Growing city populations and the process of urbanization substantially alter freshwater environments. To determine the changes in freshwater microbial communities associated with urbanization, full-length 16S rRNA gene PacBio sequencing was performed from surface water and sediments from a wastewater treatment plant, urban and rural lakes in the Berlin-Brandenburg region, Northeast Germany. Water samples exhibited highly habitat specific bacterial communities with multiple genera showing clear urban signatures. We identified potentially harmful bacterial groups associated with environmental parameters specific to urban habitats such as Alistipes, Escherichia/Shigella, Rickettsia and Streptococcus. We demonstrate that urbanization alters natural microbial communities in lakes and, via simultaneous eutrophication, creates favorable conditions that promote specific bacterial genera including potential pathogens. Our findings are of global relevance highlighting a long-term health risk in urbanized waterbodies, at a time of accelerated global urbanization. The results demonstrate the urgency for undertaking mitigation measures such as targeted lake restoration projects and sustainable water management efforts.Competing Interest StatementThe authors have declared no competing interest. ER -