PT - JOURNAL ARTICLE AU - Wang, Han AU - Okamoto, Kenta TI - A full-length infectious cDNA clone of a dsRNA totivirus-like virus AID - 10.1101/2021.11.08.467288 DP - 2021 Jan 01 TA - bioRxiv PG - 2021.11.08.467288 4099 - http://biorxiv.org/content/early/2021/11/08/2021.11.08.467288.short 4100 - http://biorxiv.org/content/early/2021/11/08/2021.11.08.467288.full AB - Totivirus-like viruses are icosahedral non-enveloped double-stranded (ds)RNA viruses belonging to a group recently discovered and provisionally assigned in the Totiviridae family. Unlike fungal and protozoan Totiviridae viruses, these totivirus-like viruses infect a diverse spectrum of metazoan hosts and currently have enormous impacts on fisheries and agriculture. We developed the first totivirus-like virus Omono River virus (OmRV) infectious full-length DNA clone and produce the infectious particles using an RNA-transcript-based method. Unlike the parent wild-type particles from nature, the reverse-genetically-generated OmRV particles had an indistinguishable cytopathic effect, infectivity, and morphology. The established system is one of the few systems that have been reported for generating a non-segmented dsRNA virus DNA clone.Competing Interest StatementThe authors have declared no competing interest.