RT Journal Article SR Electronic T1 A full-length infectious cDNA clone of a dsRNA totivirus-like virus JF bioRxiv FD Cold Spring Harbor Laboratory SP 2021.11.08.467288 DO 10.1101/2021.11.08.467288 A1 Wang, Han A1 Okamoto, Kenta YR 2021 UL http://biorxiv.org/content/early/2021/11/08/2021.11.08.467288.abstract AB Totivirus-like viruses are icosahedral non-enveloped double-stranded (ds)RNA viruses belonging to a group recently discovered and provisionally assigned in the Totiviridae family. Unlike fungal and protozoan Totiviridae viruses, these totivirus-like viruses infect a diverse spectrum of metazoan hosts and currently have enormous impacts on fisheries and agriculture. We developed the first totivirus-like virus Omono River virus (OmRV) infectious full-length DNA clone and produce the infectious particles using an RNA-transcript-based method. Unlike the parent wild-type particles from nature, the reverse-genetically-generated OmRV particles had an indistinguishable cytopathic effect, infectivity, and morphology. The established system is one of the few systems that have been reported for generating a non-segmented dsRNA virus DNA clone.Competing Interest StatementThe authors have declared no competing interest.