RT Journal Article
SR Electronic
T1 Live imaging YAP signaling in mouse embryo development
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.08.11.456026
DO 10.1101/2021.08.11.456026
A1 Bin Gu
A1 Brian Bradshaw
A1 Min Zhu
A1 Yu Sun
A1 Sevan Hopyan
A1 Janet Rossant
YR 2021
UL http://biorxiv.org/content/early/2021/11/08/2021.08.11.456026.abstract
AB YAP protein is a critical regulator of mammalian embryonic development. By generating a near-infrared fusion YAP reporter mouse line, we have achieved high-resolution live imaging of YAP localization during mouse embryonic development. We have validated the reporter by demonstrating its predicted responses to blocking LATs kinase activity or blocking cell polarity. By time lapse imaging preimplantation embryos, we revealed a mitotic reset behavior of YAP nuclear localization. We also demonstrated deep tissue live imaging in post-implantation embryos and revealed an intriguing nuclear YAP pattern in migrating cells. The YAP fusion reporter mice and imaging methods will open new opportunities for understanding dynamic YAP signaling in vivo in many different situations.Competing Interest StatementThe authors have declared no competing interest.