TY - JOUR T1 - Live imaging YAP signaling in mouse embryo development JF - bioRxiv DO - 10.1101/2021.08.11.456026 SP - 2021.08.11.456026 AU - Bin Gu AU - Brian Bradshaw AU - Min Zhu AU - Yu Sun AU - Sevan Hopyan AU - Janet Rossant Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/11/08/2021.08.11.456026.abstract N2 - YAP protein is a critical regulator of mammalian embryonic development. By generating a near-infrared fusion YAP reporter mouse line, we have achieved high-resolution live imaging of YAP localization during mouse embryonic development. We have validated the reporter by demonstrating its predicted responses to blocking LATs kinase activity or blocking cell polarity. By time lapse imaging preimplantation embryos, we revealed a mitotic reset behavior of YAP nuclear localization. We also demonstrated deep tissue live imaging in post-implantation embryos and revealed an intriguing nuclear YAP pattern in migrating cells. The YAP fusion reporter mice and imaging methods will open new opportunities for understanding dynamic YAP signaling in vivo in many different situations.Competing Interest StatementThe authors have declared no competing interest. ER -