RT Journal Article
SR Electronic
T1 An environmental control box for serial crystallography enables multi-dimensional experiments
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.11.07.467596
DO 10.1101/2021.11.07.467596
A1 Mehrabi, Pedram
A1 von Stetten, David
A1 Leimkohl, Jan-Philipp
A1 Tellkamp, Friedjof
A1 Schulz, Eike C.
YR 2021
UL http://biorxiv.org/content/early/2021/11/08/2021.11.07.467596.abstract
AB We present a new environmental enclosure for fixed-target, serial crystallography enabling full control of both the temperature and humidity. While maintaining the relative humidity to within a percent, this enclosure provides access to X-ray diffraction experiments in a wide temperature range from below 10 °C to above 80 °C. Coupled with the LAMA method, time-resolved serial crystallography experiments can now be carried out at truly physiological temperatures, providing fundamentally new insight into protein function. Using the hyperthermophile enzyme xylose isomerase, we demonstrate changes in the electron density as a function of increasing temperature and time. This method provides the necessary tools to successfully carry out multi-dimensional serial crystallography.Competing Interest StatementThe authors have declared no competing interest.