RT Journal Article
SR Electronic
T1 Single-particle tracking of dynein identifies PP2A B55/SUR-6 as a cell cycle regulator of cortical force generation
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.10.22.465443
DO 10.1101/2021.10.22.465443
A1 Alan Edwards
A1 John B. Linehan
A1 Vincent Boudreau
A1 Paul S. Maddox
YR 2021
UL http://biorxiv.org/content/early/2021/11/10/2021.10.22.465443.abstract
AB Convergence and positioning of the pronuclei and mitotic spindle of many zygotes aids efficient division and is essential for early embryonic patterning. In the C. elegans zygote, interactions between microtubules and cortically anchored dynein are key to early development. However, how cortical microtubule pulling forces are controlled through the cell cycle is less well understood. We used single-molecule imaging and a windowed mean squared displacement analysis to uncover the behavior of dynein during cortical force generation, and provide a regulatory role for protein phosphatase PP2A-B55/SUR6 via NuMA-like protein LIN-5 in this process. Previous findings and our results suggest that PP2A regulates cortical microtubule pulling forces by increasing dynein binding and unbinding to the cortical force generation complex. Our data also suggests that cortical occupancy of dynein is abrogated to vary force generation. Our approach will be broadly applicable to classify the force generation behavior of single molecules in living organisms.Competing Interest StatementThe authors have declared no competing interest.