PT  - JOURNAL ARTICLE
AU  - Sereika, Mantas
AU  - Kirkegaard, Rasmus Hansen
AU  - Karst, Søren Michael
AU  - Michaelsen, Thomas Yssing
AU  - Sørensen, Emil Aarre
AU  - Wollenberg, Rasmus Dam
AU  - Albertsen, Mads
TI  - Oxford Nanopore R10.4 long-read sequencing enables near-perfect bacterial genomes from pure cultures and metagenomes without short-read or reference polishing
AID  - 10.1101/2021.10.27.466057
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.10.27.466057
4099  - http://biorxiv.org/content/early/2021/11/10/2021.10.27.466057.short
4100  - http://biorxiv.org/content/early/2021/11/10/2021.10.27.466057.full
AB  - Long-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes. However, to obtain near-perfect genomes it has been necessary to include short-read polishing to correct insertions and deletions derived from homopolymer regions. Here, we show that Oxford Nanopore R10.4 can be used to generate near-perfect microbial genomes from isolates or metagenomes without shortread or reference polishing.Competing Interest StatementEAS, SMK, MA, RHK, and RWO are also employed at DNASense ApS. The remaining authors declare no conflict of interest.