RT Journal Article
SR Electronic
T1 Oxford Nanopore R10.4 long-read sequencing enables near-perfect bacterial genomes from pure cultures and metagenomes without short-read or reference polishing
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.10.27.466057
DO 10.1101/2021.10.27.466057
A1 Sereika, Mantas
A1 Kirkegaard, Rasmus Hansen
A1 Karst, Søren Michael
A1 Michaelsen, Thomas Yssing
A1 Sørensen, Emil Aarre
A1 Wollenberg, Rasmus Dam
A1 Albertsen, Mads
YR 2021
UL http://biorxiv.org/content/early/2021/11/10/2021.10.27.466057.abstract
AB Long-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes. However, to obtain near-perfect genomes it has been necessary to include short-read polishing to correct insertions and deletions derived from homopolymer regions. Here, we show that Oxford Nanopore R10.4 can be used to generate near-perfect microbial genomes from isolates or metagenomes without shortread or reference polishing.Competing Interest StatementEAS, SMK, MA, RHK, and RWO are also employed at DNASense ApS. The remaining authors declare no conflict of interest.