PT  - JOURNAL ARTICLE
AU  - Stephen A. Banse
AU  - Christine A. Sedore
AU  - Erik Johnson
AU  - Anna L. Coleman-Hulbert
AU  - Brian Onken
AU  - David Hall
AU  - E. Grace Jackson
AU  - Phu Huynh
AU  - Anna C. Foulger
AU  - Suzhen Guo
AU  - Theo Garrett
AU  - Jian Xue
AU  - Delaney Inman
AU  - Mackenzie L. Morshead
AU  - W. Todd Plummer
AU  - Esteban Chen
AU  - Dipa Bhaumik
AU  - Michelle K. Chen
AU  - Girish Harinath
AU  - Manish Chamoli
AU  - Rose P. Quinn
AU  - Ron Falkowski
AU  - Daniel Edgar
AU  - Madeline O. Schmidt
AU  - Mark Lucanic
AU  - Max Guo
AU  - Monica Driscoll
AU  - Gordon J. Lithgow
AU  - Patrick C. Phillips
TI  - Antioxidants green tea extract and nordihydroguaiaretic acid confer species and strain specific lifespan and health effects in <em>Caenorhabditis</em> nematodes
AID  - 10.1101/2021.11.09.464847
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.11.09.464847
4099  - http://biorxiv.org/content/early/2021/11/10/2021.11.09.464847.short
4100  - http://biorxiv.org/content/early/2021/11/10/2021.11.09.464847.full
AB  - The Caenorhabditis Intervention Testing Program (CITP) is an NIH-funded research consortium of investigators who conduct analyses at three independent sites to identify chemical interventions that reproducibly promote health and lifespan in a robust manner. The founding principle of the CITP is that compounds with positive effects across a genetically diverse panel of Caenorhabditis species and strains are likely engaging conserved biochemical pathways to exert their effects. As such, interventions that are broadly efficacious might be considered prominent compounds for translation for pre-clinical research and human clinical applications. Here, we report results generated using a recently streamlined pipeline approach for the evaluation of the effects of chemical compounds on lifespan and health. We studied five compounds previously shown to extend C. elegans lifespan or thought to promote mammalian health: 17α-estradiol, acarbose, green tea extract, nordihydroguaiaretic acid, and rapamycin. We found that green tea extract and nordihydroguaiaretic acid extend Caenorhabditis lifespan in a species-specific manner. Additionally, these two antioxidants conferred assay-specific effects in some studies—for example, decreasing survival for certain genetic backgrounds in manual survival assays in contrast with extended lifespan as assayed using automated C. elegans Lifespan Machines. We also observed that GTE and NDGA impact on older adult mobility capacity is dependent on genetic background, and that GTE reduces oxidative stress resistance in some Caenorhabditis strains. Overall, our analysis of the five compounds supports the general idea that genetic background and assay type can influence lifespan and health effects of compounds, and underscores that lifespan and health can be uncoupled by chemical interventions.Competing Interest StatementThe authors have declared no competing interest.